J Clin Monit Comput - Flow cytometry and laser scanning cytometry, a comparison of techniques.

Tópicos

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Resumo

JECTIVE: Flow and laser scanning cytometry are used extensively in research and clinical settings. These techniques provide clinicians and scientists information about cell functioning in a variety of health and disease states. An in-depth knowledge and understanding of cytometry techniques can enhance interpretation of current research findings. Our goal with this review is to reacquaint clinicians and scientists with information concerning differences between flow and laser scanning cytometry by comparing their capabilities and applications.METHODS: A Pubmed abstract search was conducted for articles on research, reviews and current texts relating to origins and use of flow and laser scanning cytometry. Attention was given to studies describing application of these techniques in the clinical setting.RESULTS: Both techniques exploit interactions between the physical properties of light. Data are immediately and automatically acquired; they are distinctly different. Flow cytometry provides valuable rapid information about a wide variety of cellular or particle characteristics. This technique does not provide the scanned high resolution image analysis needed for investigators to localize areas of interest within the cell for quantification. Flow cytometry requires that the sample contain a large amount disaggregated, single, suspended cells. Laser scanning cytometry is slide-based and does not require as large of a sample. The tissue sample is affixed to a slide allowing repeated sample analyses. These cytometry techniques are used in the clinical setting to understand pathophysiological derangements associated with many diseases; cardiovascular disease, diabetes, acute lung injury, hemorrhagic shock, surgery, cancer and Alzheimer's disease.CONCLUSIONS: Understanding the differences between FCM and LSCM can assist investigators in planning and design of their research or clinical testing. Researchers and clinicians optimize these technique capabilities with the cellular characteristics they wish to measure delineating molecular and cellular events occurring in health and disease. Discovery of mechanisms in cells using FCM and LSCM provide evidence needed to guide future treatment and interventions.

Resumo Limpo

jectiv flow laser scan cytometri use extens research clinic set techniqu provid clinician scientist inform cell function varieti health diseas state indepth knowledg understand cytometri techniqu can enhanc interpret current research find goal review reacquaint clinician scientist inform concern differ flow laser scan cytometri compar capabl applicationsmethod pubm abstract search conduct articl research review current text relat origin use flow laser scan cytometri attent given studi describ applic techniqu clinic settingresult techniqu exploit interact physic properti light data immedi automat acquir distinct differ flow cytometri provid valuabl rapid inform wide varieti cellular particl characterist techniqu provid scan high resolut imag analysi need investig local area interest within cell quantif flow cytometri requir sampl contain larg amount disaggreg singl suspend cell laser scan cytometri slidebas requir larg sampl tissu sampl affix slide allow repeat sampl analys cytometri techniqu use clinic set understand pathophysiolog derang associ mani diseas cardiovascular diseas diabet acut lung injuri hemorrhag shock surgeri cancer alzheim diseaseconclus understand differ fcm lscm can assist investig plan design research clinic test research clinician optim techniqu capabl cellular characterist wish measur delin molecular cellular event occur health diseas discoveri mechan cell use fcm lscm provid evid need guid futur treatment intervent

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