J. Comput. Biol. - A new method for quantitative real-time polymerase chain reaction data analysis.

Tópicos

{ method(984) reconstruct(947) comput(926) }
{ imag(2830) propos(1344) filter(1198) }
{ inform(2794) health(2639) internet(1427) }
{ result(1111) use(1088) new(759) }
{ data(3008) multipl(1320) sourc(1022) }
{ method(2212) result(1239) propos(1039) }
{ algorithm(1844) comput(1787) effici(935) }
{ error(1145) method(1030) estim(1020) }
{ general(901) number(790) one(736) }
{ perform(1367) use(1326) method(1137) }
{ model(3480) simul(1196) paramet(876) }
{ estim(2440) model(1874) function(577) }
{ detect(2391) sensit(1101) algorithm(908) }
{ sequenc(1873) structur(1644) protein(1328) }
{ studi(1410) differ(1259) use(1210) }
{ research(1085) discuss(1038) issu(1018) }
{ time(1939) patient(1703) rate(768) }
{ drug(1928) target(777) effect(648) }
{ can(774) often(719) complex(702) }
{ imag(1947) propos(1133) code(1026) }
{ bind(1733) structur(1185) ligand(1036) }
{ take(945) account(800) differ(722) }
{ assess(1506) score(1403) qualiti(1306) }
{ problem(2511) optim(1539) algorithm(950) }
{ concept(1167) ontolog(924) domain(897) }
{ clinic(1479) use(1117) guidelin(835) }
{ control(1307) perform(991) simul(935) }
{ visual(1396) interact(850) tool(830) }
{ compound(1573) activ(1297) structur(1058) }
{ monitor(1329) mobil(1314) devic(1160) }
{ gene(2352) biolog(1181) express(1162) }
{ analysi(2126) use(1163) compon(1037) }
{ process(1125) use(805) approach(778) }
{ model(3404) distribut(989) bayesian(671) }
{ data(1737) use(1416) pattern(1282) }
{ system(1976) rule(880) can(841) }
{ measur(2081) correl(1212) valu(896) }
{ imag(1057) registr(996) error(939) }
{ method(1219) similar(1157) match(930) }
{ featur(3375) classif(2383) classifi(1994) }
{ network(2748) neural(1063) input(814) }
{ imag(2675) segment(2577) method(1081) }
{ patient(2315) diseas(1263) diabet(1191) }
{ studi(2440) review(1878) systemat(933) }
{ motion(1329) object(1292) video(1091) }
{ treatment(1704) effect(941) patient(846) }
{ surgeri(1148) surgic(1085) robot(1054) }
{ framework(1458) process(801) describ(734) }
{ chang(1828) time(1643) increas(1301) }
{ learn(2355) train(1041) set(1003) }
{ extract(1171) text(1153) clinic(932) }
{ method(1557) propos(1049) approach(1037) }
{ data(1714) softwar(1251) tool(1186) }
{ design(1359) user(1324) use(1319) }
{ model(2220) cell(1177) simul(1124) }
{ care(1570) inform(1187) nurs(1089) }
{ search(2224) databas(1162) retriev(909) }
{ featur(1941) imag(1645) propos(1176) }
{ case(1353) use(1143) diagnosi(1136) }
{ howev(809) still(633) remain(590) }
{ data(3963) clinic(1234) research(1004) }
{ risk(3053) factor(974) diseas(938) }
{ perform(999) metric(946) measur(919) }
{ system(1050) medic(1026) inform(1018) }
{ import(1318) role(1303) understand(862) }
{ model(2341) predict(2261) use(1141) }
{ studi(1119) effect(1106) posit(819) }
{ blood(1257) pressur(1144) flow(957) }
{ spatial(1525) area(1432) region(1030) }
{ record(1888) medic(1808) patient(1693) }
{ health(3367) inform(1360) care(1135) }
{ ehr(2073) health(1662) electron(1139) }
{ state(1844) use(1261) util(961) }
{ research(1218) medic(880) student(794) }
{ patient(2837) hospit(1953) medic(668) }
{ model(2656) set(1616) predict(1553) }
{ data(2317) use(1299) case(1017) }
{ age(1611) year(1155) adult(843) }
{ medic(1828) order(1363) alert(1069) }
{ signal(2180) analysi(812) frequenc(800) }
{ cost(1906) reduc(1198) effect(832) }
{ group(2977) signific(1463) compar(1072) }
{ sampl(1606) size(1419) use(1276) }
{ first(2504) two(1366) second(1323) }
{ intervent(3218) particip(2042) group(1664) }
{ activ(1138) subject(705) human(624) }
{ patient(1821) servic(1111) care(1106) }
{ use(2086) technolog(871) perceiv(783) }
{ can(981) present(881) function(850) }
{ health(1844) social(1437) communiti(874) }
{ structur(1116) can(940) graph(676) }
{ high(1669) rate(1365) level(1280) }
{ cancer(2502) breast(956) screen(824) }
{ use(976) code(926) identifi(902) }
{ use(1733) differ(960) four(931) }
{ implement(1333) system(1263) develop(1122) }
{ survey(1388) particip(1329) question(1065) }
{ decis(3086) make(1611) patient(1517) }
{ activ(1452) weight(1219) physic(1104) }
{ method(1969) cluster(1462) data(1082) }

Resumo

Quantitative real-time polymerase chain reaction (qPCR) is a sensitive gene quantification method that has been extensively used in biological and biomedical fields. The currently used methods for PCR data analysis, including the threshold cycle method and linear and nonlinear model-fitting methods, all require subtracting background fluorescence. However, the removal of background fluorescence can hardly be accurate and therefore can distort results. We propose a new method, the taking-difference linear regression method, to overcome this limitation. Briefly, for each two consecutive PCR cycles, we subtract the fluorescence in the former cycle from that in the latter cycle, transforming the n cycle raw data into n-1 cycle data. Then, linear regression is applied to the natural logarithm of the transformed data. Finally, PCR amplification efficiencies and the initial DNA molecular numbers are calculated for each reaction. This taking-difference method avoids the error in subtracting an unknown background, and thus it is more accurate and reliable. This method is easy to perform, and this strategy can be extended to all current methods for PCR data analysis.

Resumo Limpo

quantit realtim polymeras chain reaction qpcr sensit gene quantif method extens use biolog biomed field current use method pcr data analysi includ threshold cycl method linear nonlinear modelfit method requir subtract background fluoresc howev remov background fluoresc can hard accur therefor can distort result propos new method takingdiffer linear regress method overcom limit briefli two consecut pcr cycl subtract fluoresc former cycl latter cycl transform n cycl raw data n cycl data linear regress appli natur logarithm transform data final pcr amplif effici initi dna molecular number calcul reaction takingdiffer method avoid error subtract unknown background thus accur reliabl method easi perform strategi can extend current method pcr data analysi

Resumos Similares

Comput. Biol. Med. - Expectation maximization (EM) algorithms using polar symmetries for computed tomography (CT) image reconstruction. ( 0,715670707866384 )
Comput Methods Programs Biomed - Comparison of algorithms for out-of-plane artifacts removal in digital tomosynthesis reconstructions. ( 0,702361098823356 )
IEEE Trans Image Process - Fast approximation of algebraic reconstruction methods for tomography. ( 0,663697194065169 )
Comput Math Methods Med - A novel CT imaging system with adjacent double X-ray sources. ( 0,662466695378669 )
IEEE Trans Image Process - Interpolation-based image super-resolution using multisurface fitting. ( 0,657870976377007 )
Comput Math Methods Med - Plane-based sampling for ray casting algorithm in sequential medical images. ( 0,646036548412316 )
IEEE Trans Vis Comput Graph - Surface Meshing with Curvature Convergence. ( 0,645053040495114 )
J Med Syst - Analysis of enlarged images using time-of-flight magnetic resonance angiography, computed tomography, and conventional angiography. ( 0,640270170643607 )
IEEE Trans Vis Comput Graph - A Curvature-Adaptive Implicit Surface Reconstruction for Irregularly Spaced Points. ( 0,638751048025727 )
IEEE Trans Image Process - Stereo matching using epipolar distance transform. ( 0,635261722127575 )
Comput. Biol. Med. - Tooth model reconstruction based upon data fusion for orthodontic treatment simulation. ( 0,632701509698466 )
IEEE Trans Vis Comput Graph - Approximate Boolean Operations on Large Polyhedral Solids with Partial Mesh Reconstruction. ( 0,630755342851493 )
Int J Med Robot - Dynamic cylindrical free-form deformation for interactive simulation of tool-tissue interaction. ( 0,619548893332653 )
Comput Math Methods Med - Low-dose and scatter-free cone-beam CT imaging using a stationary beam blocker in a single scan: phantom studies. ( 0,617153355352793 )
IEEE Trans Vis Comput Graph - Image-Space Texture-Based Output-Coherent Surface Flow Visualization. ( 0,613384507772618 )
Int J Comput Assist Radiol Surg - Accurate image reconstruction using real C-arm data from a Circle-plus-arc trajectory. ( 0,613048607816759 )
IEEE Trans Image Process - 3-D reconstruction of microtubules from multi-angle total internal reflection fluorescence microscopy using Bayesian framework. ( 0,60632967267269 )
IEEE Trans Image Process - DART: a practical reconstruction algorithm for discrete tomography. ( 0,606165348468094 )
Comput Math Methods Med - A novel blind separation method in magnetic resonance images. ( 0,605321440491711 )
IEEE Trans Image Process - Efficient volume reconstruction for parallel-beam computed laminography by filtered backprojection on multi-core clusters. ( 0,604388098901082 )
IEEE Trans Image Process - Lookup-table-based gradient field reconstruction. ( 0,603210666285095 )
Comput Math Methods Med - The X-ray transform projection of 3D mother wavelet function. ( 0,60015494262135 )
IEEE Trans Vis Comput Graph - Approximation of Loop Subdivision Surfaces for Fast Rendering. ( 0,598101010206235 )
Comput. Aided Surg. - Mean-shifted surface curvature algorithm for automatic bone shape segmentation in orthopedic surgery planning: a sensitivity analysis. ( 0,597880997564667 )
Comput Math Methods Med - Piecewise-constant-model-based interior tomography applied to dentin tubules. ( 0,597816355312388 )
IEEE Trans Image Process - Higher degree total variation (HDTV) regularization for image recovery. ( 0,593531955275772 )
Med Biol Eng Comput - Fast 3D reconstruction of the rib cage from biplanar radiographs. ( 0,591957525151868 )
IEEE Trans Vis Comput Graph - Linear Correlations between Spatial and Normal Noise in Triangle Meshes. ( 0,591136514844207 )
IEEE Trans Image Process - Detection and restoration of defective lines in the SPOT 4 SWIR band. ( 0,587008126935948 )
IEEE Trans Image Process - Piecewise linear curve approximation using graph theory and geometrical concepts. ( 0,586183065963388 )
IEEE Trans Image Process - Improving shape retrieval by spectral matching and meta similarity. ( 0,585572486356826 )
Comput Math Methods Med - Automatic vasculature identification in coronary angiograms by adaptive geometrical tracking. ( 0,584789545735657 )
IEEE Trans Vis Comput Graph - Property and Lighting Manipulations for Static Volume Stylization Using a Painting Metaphor. ( 0,584205602035108 )
IEEE Trans Image Process - Multi-view video representation based on fast Monte Carlo surface reconstruction. ( 0,582006763321641 )
IEEE Trans Image Process - Efficient halftoning based on multiple look-up tables. ( 0,579225767567704 )
IEEE Trans Vis Comput Graph - Real-Time Shape Illustration Using Laplacian Lines. ( 0,57728767723554 )
J Chem Inf Model - Improving protocols for protein mapping through proper comparison to crystallography data. ( 0,577100539401919 )
Comput Methods Programs Biomed - Fast computation of Hessian-based enhancement filters for medical images. ( 0,576259095234515 )
Int J Comput Assist Radiol Surg - Successive iterative restoration applied to streak artifact reduction in X-ray CT image of dento-alveolar region. ( 0,573244644538788 )
IEEE Trans Pattern Anal Mach Intell - Dynamic Refraction Stereo. ( 0,572561759092396 )
IEEE Trans Image Process - A model based iterative reconstruction algorithm for high angle annular dark field-scanning transmission electron microscope (HAADF-STEM) tomography. ( 0,572129567690623 )
IEEE Trans Image Process - Simulation of fractional Brownian surfaces via spectral synthesis on manifolds. ( 0,57176619542933 )
Comput Math Methods Med - Fitting C? continuous parametric surfaces to frontiers delimiting physiologic structures. ( 0,570513882534426 )
IEEE Trans Image Process - Missing intensity interpolation using a kernel PCA-based POCS algorithm and its applications. ( 0,569571328646003 )
IEEE Trans Image Process - Content-aware dark image enhancement through channel division. ( 0,568438055228776 )
Comput Math Methods Med - 3D alternating direction TV-based cone-beam CT reconstruction with efficient GPU implementation. ( 0,566608353827663 )
IEEE Trans Image Process - Accelerated edge-preserving image restoration without boundary artifacts. ( 0,566340503304604 )
IEEE Trans Image Process - Optimized 3D watermarking for minimal surface distortion. ( 0,566133215330764 )
Med Biol Eng Comput - Three-dimensional reconstruction of cranial defect using active contour model and image registration. ( 0,565814050252494 )
IEEE Trans Image Process - Improving filtered backprojection reconstruction by data-dependent filtering. ( 0,563530628966149 )
IEEE Trans Image Process - Shape-from-focus by tensor voting. ( 0,563051732230337 )
IEEE Trans Image Process - Adaptive reconstruction of millimeter-wave radiometric images. ( 0,562079807715283 )
IEEE Trans Vis Comput Graph - The Sinogram Polygonizer for Reconstructing 3D Shapes. ( 0,561386883964087 )
Comput Math Methods Med - Hybrid mesh for nasal airflow studies. ( 0,5607750523815 )
IEEE Trans Image Process - Efficient reconstruction of dielectric objects based on integral equation approach with Gauss-Newton minimization. ( 0,558552401589627 )
IEEE Trans Pattern Anal Mach Intell - On Differential Photometric Reconstruction for Unknown, Isotropic BRDFs. ( 0,55851840317732 )
Comput Methods Programs Biomed - Fast ray-tracing of human eye optics on Graphics Processing Units. ( 0,558161863162381 )
IEEE Trans Image Process - Implicit B-spline surface reconstruction. ( 0,557773997119801 )
IEEE Trans Image Process - Segment adaptive gradient angle interpolation. ( 0,557194945920815 )
IEEE Trans Image Process - Linearly estimating all parameters of affine motion using Radon transform. ( 0,556472792898662 )
IEEE Trans Image Process - Accelerating x-ray data collection using pyramid beam ray casting geometries. ( 0,556131730960709 )
Comput Math Methods Med - Distributed reconstruction via alternating direction method. ( 0,555820796627861 )
J Chem Inf Model - Economical and accurate protocol for calculating hydrogen-bond-acceptor strengths. ( 0,555633188214366 )
IEEE Trans Vis Comput Graph - Interactive Visualization of Rotational Symmetry Fields on Surfaces. ( 0,555440664834058 )
IEEE Trans Vis Comput Graph - Translucent Radiosity: Efficiently Combining Diffuse Inter-reflection and Subsurface Scattering. ( 0,554851949722128 )
IEEE Trans Image Process - A PDE formalization of Retinex theory. ( 0,55289577398405 )
IEEE Trans Image Process - Image integrity authentication scheme based on fixed point theory. ( 0,550883061977687 )
Comput Math Methods Med - A CT reconstruction algorithm based on non-aliasing Contourlet transform and compressive sensing. ( 0,550614280981494 )
J Chem Inf Model - Kernel density estimation applied to bond length, bond angle, and torsion angle distributions. ( 0,550612004745015 )
Comput Math Methods Med - A CT Reconstruction Algorithm Based on L1/2 Regularization. ( 0,549688694509739 )
Comput Methods Programs Biomed - Evaluating iterative algebraic algorithms in terms of convergence and image quality for cone beam CT. ( 0,549456489950972 )
IEEE Trans Image Process - Single image super-resolution with non-local means and steering kernel regression. ( 0,549335191693544 )
Med Biol Eng Comput - A hybrid approach for the control of axonal outgrowth: preliminary simulation results. ( 0,549025494474755 )
Comput. Biol. Med. - A femur fracture reduction method based on anatomy of the contralateral side. ( 0,54785202744138 )
IEEE Trans Image Process - Parametric polytope reconstruction, an application to crystal shape estimation. ( 0,54722588316154 )
Comput. Biol. Med. - Analytical cone-beam SPECT reconstruction algorithm with non-uniform attenuation for general non-circular orbit. ( 0,544805400918328 )
IEEE Trans Pattern Anal Mach Intell - As-Projective-As-Possible Image Stitching with Moving DLT. ( 0,544117477928465 )
J. Comput. Biol. - Shapes of interacting RNA complexes. ( 0,543781356409907 )
Comput. Biol. Med. - Cascaded-Automatic Segmentation for Schistosoma japonicum eggs in images of fecal samples. ( 0,543588875759301 )
IEEE Trans Vis Comput Graph - Cone Tracing for Furry Object Rendering. ( 0,543234266570663 )
IEEE Trans Image Process - A multiresolution approach to iterative reconstruction algorithms in X-ray computed tomography. ( 0,542493688591747 )
IEEE Trans Image Process - Multiclass maximum-likelihood symmetry determination and motif reconstruction of 3-D helical objects from projection images for electron microscopy. ( 0,542060422854302 )
IEEE Trans Vis Comput Graph - Lattice Cleaving: A Multimaterial Tetrahedral Meshing Algorithm with Guarantees. ( 0,541158036142199 )
J Chem Inf Model - Characterization of biaryl torsional energetics and its treatment in OPLS all-atom force fields. ( 0,539279479779937 )
Comput. Biol. Med. - Generalized Gibbs priors based positron emission tomography reconstruction. ( 0,538996943305664 )
IEEE Trans Image Process - Fast space-variant elliptical filtering using box splines. ( 0,538232552043162 )
IEEE J Biomed Health Inform - An Adaptive Filter for the Removal of Drifting Sinusoidal Noise without a Reference. ( 0,537893749138728 )
IEEE J Biomed Health Inform - Part-based multiderivative edge cross-sectional profiles for polyp detection in colonoscopy. ( 0,535649722007379 )
Int J Med Robot - Comparison of different reconstruction algorithms for three-dimensional ultrasound imaging in a neurosurgical setting. ( 0,535339031207171 )
IEEE Trans Image Process - Filtering chromatic aberration for wide acceptance angle electrostatic lenses. ( 0,533181965063922 )
IEEE Trans Image Process - An improved image compression algorithm using binary space partition scheme and geometric wavelets. ( 0,531652142732811 )
J Med Syst - Research on interpolation methods in medical image processing. ( 0,530838246954336 )
IEEE Trans Vis Comput Graph - Precomputed Safety Shapes for Efficient and Accurate Height-Field Rendering. ( 0,530528643174459 )
IEEE Trans Image Process - ViBe: a universal background subtraction algorithm for video sequences. ( 0,529112202253379 )
IEEE Trans Vis Comput Graph - Data-Parallel Octrees for Surface Reconstruction. ( 0,527943870810502 )
J Med Syst - A new method for 3D thinning of hybrid shaped porous media using artificial intelligence. Application to trabecular bone. ( 0,527572534591519 )
IEEE Trans Image Process - Nonlinear image upsampling method based on radial basis function interpolation. ( 0,527156479476376 )
IEEE Trans Pattern Anal Mach Intell - Robustly Aligning a Shape Model and Its Application to Car Alignment of Unknown Pose. ( 0,527134138312231 )
Comput. Biol. Med. - Evaluation of robust wave image processing methods for magnetic resonance elastography. ( 0,52677746481046 )
Int J Comput Assist Radiol Surg - MR image reconstruction based on framelets and nonlocal total variation using split Bregman method. ( 0,526454770143195 )