Med Biol Eng Comput - Automated tracking and analysis of phospholipid vesicle contours in phase contrast microscopy images.

Tópicos

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Resumo

In this article, we propose a method for automated tracking and analysis of vesicle contours in video sequences acquired by phase contrast microscopy. The contour is determined in each frame of the selected video sequence by detecting the transition between the interior and exterior of the vesicle that is reflected in the image intensity gradients. The resulting contour points are represented in the polar coordinate system, i.e., with uniform angular sampling and with coordinates that originate from the vesicle center of mass, enabling the analysis of the vesicle shape and its membrane fluctuations. By analyzing artificial images with known ground-truth contours, the accuracy and precision of the proposed method was estimated to be 34.1 and 26.9 nm for image signal-to-noise ratio of 23 dB and pixel size of 35 nm, respectively. The proposed method was evaluated on quasi-spherical vesicles made up of different proportions of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and cholesterol and exposed to different temperatures. The results show that the method is robust and efficient in terms of speed and quantitative description of vesicle fluctuations. The magnitude of vesicle membrane fluctuations increased with temperature, while the bending rigidity of the membrane was increasing for temperatures up to 20 ?C and decreasing for higher temperatures irrespective of the vesicle molecular structure.

Resumo Limpo

articl propos method autom track analysi vesicl contour video sequenc acquir phase contrast microscopi contour determin frame select video sequenc detect transit interior exterior vesicl reflect imag intens gradient result contour point repres polar coordin system ie uniform angular sampl coordin origin vesicl center mass enabl analysi vesicl shape membran fluctuat analyz artifici imag known groundtruth contour accuraci precis propos method estim nm imag signaltonois ratio db pixel size nm respect propos method evalu quasispher vesicl made differ proport palmitoyloleoylsnglycerophosphocholin popc cholesterol expos differ temperatur result show method robust effici term speed quantit descript vesicl fluctuat magnitud vesicl membran fluctuat increas temperatur bend rigid membran increas temperatur c decreas higher temperatur irrespect vesicl molecular structur

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