Med Biol Eng Comput - Electric impedance of human embryonic stem cell-derived retinal pigment epithelium.

Tópicos

{ model(2220) cell(1177) simul(1124) }
{ can(981) present(881) function(850) }
{ perform(1367) use(1326) method(1137) }
{ model(3480) simul(1196) paramet(876) }
{ bind(1733) structur(1185) ligand(1036) }
{ assess(1506) score(1403) qualiti(1306) }
{ system(1050) medic(1026) inform(1018) }
{ visual(1396) interact(850) tool(830) }
{ activ(1138) subject(705) human(624) }
{ sequenc(1873) structur(1644) protein(1328) }
{ problem(2511) optim(1539) algorithm(950) }
{ gene(2352) biolog(1181) express(1162) }
{ use(1733) differ(960) four(931) }
{ can(774) often(719) complex(702) }
{ imag(1947) propos(1133) code(1026) }
{ data(1737) use(1416) pattern(1282) }
{ studi(2440) review(1878) systemat(933) }
{ motion(1329) object(1292) video(1091) }
{ chang(1828) time(1643) increas(1301) }
{ clinic(1479) use(1117) guidelin(835) }
{ algorithm(1844) comput(1787) effici(935) }
{ control(1307) perform(991) simul(935) }
{ studi(1119) effect(1106) posit(819) }
{ blood(1257) pressur(1144) flow(957) }
{ state(1844) use(1261) util(961) }
{ data(2317) use(1299) case(1017) }
{ use(2086) technolog(871) perceiv(783) }
{ structur(1116) can(940) graph(676) }
{ high(1669) rate(1365) level(1280) }
{ estim(2440) model(1874) function(577) }
{ activ(1452) weight(1219) physic(1104) }
{ model(3404) distribut(989) bayesian(671) }
{ inform(2794) health(2639) internet(1427) }
{ system(1976) rule(880) can(841) }
{ measur(2081) correl(1212) valu(896) }
{ imag(1057) registr(996) error(939) }
{ method(1219) similar(1157) match(930) }
{ featur(3375) classif(2383) classifi(1994) }
{ imag(2830) propos(1344) filter(1198) }
{ network(2748) neural(1063) input(814) }
{ imag(2675) segment(2577) method(1081) }
{ patient(2315) diseas(1263) diabet(1191) }
{ take(945) account(800) differ(722) }
{ treatment(1704) effect(941) patient(846) }
{ surgeri(1148) surgic(1085) robot(1054) }
{ framework(1458) process(801) describ(734) }
{ error(1145) method(1030) estim(1020) }
{ learn(2355) train(1041) set(1003) }
{ concept(1167) ontolog(924) domain(897) }
{ extract(1171) text(1153) clinic(932) }
{ method(1557) propos(1049) approach(1037) }
{ data(1714) softwar(1251) tool(1186) }
{ design(1359) user(1324) use(1319) }
{ care(1570) inform(1187) nurs(1089) }
{ general(901) number(790) one(736) }
{ method(984) reconstruct(947) comput(926) }
{ search(2224) databas(1162) retriev(909) }
{ featur(1941) imag(1645) propos(1176) }
{ case(1353) use(1143) diagnosi(1136) }
{ howev(809) still(633) remain(590) }
{ data(3963) clinic(1234) research(1004) }
{ studi(1410) differ(1259) use(1210) }
{ risk(3053) factor(974) diseas(938) }
{ perform(999) metric(946) measur(919) }
{ research(1085) discuss(1038) issu(1018) }
{ import(1318) role(1303) understand(862) }
{ model(2341) predict(2261) use(1141) }
{ compound(1573) activ(1297) structur(1058) }
{ spatial(1525) area(1432) region(1030) }
{ record(1888) medic(1808) patient(1693) }
{ health(3367) inform(1360) care(1135) }
{ monitor(1329) mobil(1314) devic(1160) }
{ ehr(2073) health(1662) electron(1139) }
{ research(1218) medic(880) student(794) }
{ patient(2837) hospit(1953) medic(668) }
{ model(2656) set(1616) predict(1553) }
{ age(1611) year(1155) adult(843) }
{ medic(1828) order(1363) alert(1069) }
{ signal(2180) analysi(812) frequenc(800) }
{ cost(1906) reduc(1198) effect(832) }
{ group(2977) signific(1463) compar(1072) }
{ sampl(1606) size(1419) use(1276) }
{ data(3008) multipl(1320) sourc(1022) }
{ first(2504) two(1366) second(1323) }
{ intervent(3218) particip(2042) group(1664) }
{ time(1939) patient(1703) rate(768) }
{ patient(1821) servic(1111) care(1106) }
{ analysi(2126) use(1163) compon(1037) }
{ health(1844) social(1437) communiti(874) }
{ cancer(2502) breast(956) screen(824) }
{ use(976) code(926) identifi(902) }
{ drug(1928) target(777) effect(648) }
{ result(1111) use(1088) new(759) }
{ implement(1333) system(1263) develop(1122) }
{ survey(1388) particip(1329) question(1065) }
{ decis(3086) make(1611) patient(1517) }
{ process(1125) use(805) approach(778) }
{ method(1969) cluster(1462) data(1082) }
{ method(2212) result(1239) propos(1039) }
{ detect(2391) sensit(1101) algorithm(908) }

Resumo

The barrier properties of epithelium are conventionally defined by transepithelial resistance (TER). TER provides information about the tightness of the epithelium. Electrical impedance spectroscopy (EIS) provides additional information regarding cell membrane properties, such as changes in electric capacitance and possible parallel or serial pathways that may correlate with the morphology of the cell layer. This study presents EIS of retinal pigment epithelial (RPE) cell model of the putative RPE differentiated from human embryonic stem cells (hESC-RPE). The generally utilized RPE cell model, ARPE-19, was used as immature control. The measured EIS was analyzed by fitting an equivalent electrical circuit model describing the resistive and capacitive properties of the RPE. Our results indicated that TER of hESC-RPE cells was close to the values of human RPE presented in the literature. This provides evidence that the stem cell-derived RPE in vitro can reach high-barrier function. Furthermore, hESC-RPE cells produced impedance spectra that can be modeled by the equivalent circuit of one time constant. ARPE-19 cells produced low-barrier properties, that is, an impedance spectra that suggested poor maturation of ARPE-19 cells. To conclude, EIS could give us means for non-invasively estimating the functionality and maturation of differentiated-RPE cells.

Resumo Limpo

barrier properti epithelium convent defin transepitheli resist ter ter provid inform tight epithelium electr imped spectroscopi ei provid addit inform regard cell membran properti chang electr capacit possibl parallel serial pathway may correl morpholog cell layer studi present ei retin pigment epitheli rpe cell model putat rpe differenti human embryon stem cell hescrp general util rpe cell model arp use immatur control measur ei analyz fit equival electr circuit model describ resist capacit properti rpe result indic ter hescrp cell close valu human rpe present literatur provid evid stem cellderiv rpe vitro can reach highbarri function furthermor hescrp cell produc imped spectra can model equival circuit one time constant arp cell produc lowbarri properti imped spectra suggest poor matur arp cell conclud ei give us mean noninvas estim function matur differentiatedrp cell

Resumos Similares

Comput. Biol. Med. - Mathematical model of the coagulation in the bone-dental implant interface. ( 0,813342388106007 )
Comput Methods Programs Biomed - A phenomenological mathematical model of the articular cartilage damage. ( 0,788661026582787 )
Comput. Biol. Med. - Prediction of stress shielding around an orthopedic screw: using stress and strain energy density as mechanical stimuli. ( 0,778568579356045 )
Comput Math Methods Med - Quantitative imaging of young's modulus of soft tissues from ultrasound water jet indentation: a finite element study. ( 0,776957102506227 )
Comput Math Methods Med - The influence of the specimen shape and loading conditions on the parameter identification of a viscoelastic brain model. ( 0,771185835105349 )
Comput. Biol. Med. - Reconstruction of the biomechanical transfer path of femoral head necrosis: a subject-specific finite element investigation. ( 0,763306457305367 )
Comput Math Methods Med - Comparative evaluation of osseointegrated dental implants based on platform-switching concept: influence of diameter, length, thread shape, and in-bone positioning depth on stress-based performance. ( 0,75363387237148 )
Med Biol Eng Comput - Mathematical modeling of stem cell proliferation. ( 0,745761124990818 )
Med Biol Eng Comput - Effects of simulated injury on the anteroinferior glenohumeral capsule. ( 0,744269620163404 )
IEEE J Biomed Health Inform - Quantitative deconvolution of human thermal infrared emittance. ( 0,743556746605643 )
Comput Methods Programs Biomed - Experiment and hydro-mechanical coupling simulation study on the human periodontal ligament. ( 0,740341034029492 )
Int J Comput Assist Radiol Surg - Surgical stent planning: simulation parameter study for models based on DICOM standards. ( 0,730726792092781 )
Comput Math Methods Med - Contribution to the determination of in vivo mechanical characteristics of human skin by indentation test. ( 0,730637586599915 )
Med Biol Eng Comput - Clinically oriented real-time monitoring of the individual's risk for deep tissue injury. ( 0,729446463079741 )
Comput Methods Programs Biomed - Growth plate stress distribution implications during bone development: a simple framework computational approach. ( 0,728230461051171 )
Med Biol Eng Comput - Mechanical and microarchitectural analyses of cancellous bone through experiment and computer simulation. ( 0,726823824575133 )
Med Biol Eng Comput - Temporal evolution of skeletal regenerated tissue: what can mechanical investigation add to biological? ( 0,723332520887581 )
Med Biol Eng Comput - Finite element modeling of mitral leaflet tissue using a layered shell approximation. ( 0,72315581694906 )
Comput. Biol. Med. - Computational studies on strain transmission from a collagen gel construct to a cell and its internal cytoskeletal filaments. ( 0,722505876095946 )
Comput. Biol. Med. - Biological-data-based finite-element stress analysis of mandibular bone with implant-supported overdenture. ( 0,719943085279641 )
Med Biol Eng Comput - Multi-scale study of nanoparticle transport and deposition in tissues during an injection process. ( 0,719274530681558 )
Comput Math Methods Med - A finite element study of micropipette aspiration of single cells: effect of compressibility. ( 0,719220482372612 )
Comput. Biol. Med. - Protein transport in the connecting cilium of a photoreceptor cell: modeling the effects of bidirectional protein transitions between the diffusion-driven and motor-driven kinetic states. ( 0,7172297767714 )
Comput Methods Programs Biomed - Subject-specific finite element simulation of the human femur considering inhomogeneous material properties: a straightforward method and convergence study. ( 0,716170490660885 )
Comput Methods Programs Biomed - Modelling of mandible bone properties in the numerical analysis of oral implant biomechanics. ( 0,715201261280513 )
Med Biol Eng Comput - A three-dimensional finite element model of the cervical spine: an investigation of whiplash injury. ( 0,713490110151927 )
Comput Math Methods Med - Dynamics of posttranslational modifications of p53. ( 0,713331410313843 )
Med Biol Eng Comput - 3D finite element analysis on esthetic indirect dental restorations under thermal and mechanical loading. ( 0,712026106278548 )
Med Biol Eng Comput - Calibration of the mechanical properties in a finite element model of a lumbar vertebra under dynamic compression up to failure. ( 0,708827346655285 )
Med Biol Eng Comput - Sensitivity studies of pediatric material properties on juvenile lumbar spine responses using finite element analysis. ( 0,70870537743374 )
Comput Math Methods Med - Fluid structural analysis of human cerebral aneurysm using their own wall mechanical properties. ( 0,708276855244689 )
Int J Med Robot - A physics-based algorithm for real-time simulation of electrosurgery procedures in minimally invasive surgery. ( 0,706295293161716 )
Comput Math Methods Med - Superficial collagen fibril modulus and pericellular fixed charge density modulate chondrocyte volumetric behaviour in early osteoarthritis. ( 0,705862968823065 )
Comput Math Methods Med - Study of tumor growth under hyperthermia condition. ( 0,703840336991684 )
Med Biol Eng Comput - Modeling and analysis of coagulated liver tissue and its interaction with a scalpel blade. ( 0,702575817749802 )
Int J Comput Assist Radiol Surg - Bivariate optimization of orthodontic mini-implant thread height and pitch. ( 0,701091748803556 )
Med Biol Eng Comput - In-silico modeling of atrial repolarization in normal and atrial fibrillation remodeled state. ( 0,698032554650557 )
Comput Math Methods Med - Morphological measurement of living cells in methanol with digital holographic microscopy. ( 0,697403773662273 )
Med Biol Eng Comput - A new method to include the gravitational forces in a finite element model of the scoliotic spine. ( 0,696256867060309 )
Comput Methods Programs Biomed - HiL simulation in biomechanics: a new approach for testing total joint replacements. ( 0,693677121247806 )
Comput Math Methods Med - A literature review of the numerical analysis of abdominal aortic aneurysms treated with endovascular stent grafts. ( 0,692741508078525 )
Comput Math Methods Med - A review of the combination of experimental measurements and fibril-reinforced modeling for investigation of articular cartilage and chondrocyte response to loading. ( 0,69145524055609 )
Comput Math Methods Med - Recent advances in computational mechanics of the human knee joint. ( 0,691405040719445 )
Med Biol Eng Comput - Modeling and experimental methods to predict oxygen distribution in bone defects following cell transplantation. ( 0,69103309318347 )
Med Biol Eng Comput - Influence of ischemic core muscle fibers on surface depolarization potentials in superfused cardiac tissue preparations: a simulation study. ( 0,690033835720409 )
Med Biol Eng Comput - Finite element analysis of the influence of loading rate on a model of the full lumbar spine under dynamic loading conditions. ( 0,689473836248602 )
Med Biol Eng Comput - Sensitivity of patient-specific vertebral finite element model from low dose imaging to material properties and loading conditions. ( 0,688069509532588 )
Comput Math Methods Med - Scaffold library for tissue engineering: a geometric evaluation. ( 0,687789372955938 )
Med Biol Eng Comput - Change of mechanical vertebrae properties due to progressive osteoporosis: combined biomechanical and finite-element analysis within a rat model. ( 0,686397934341911 )
Med Biol Eng Comput - Biomechanical characterisation of fresh and cadaverous human small intestine: applications for abdominal trauma. ( 0,685375750839766 )
J Med Syst - Post-operative assessment of an implant fixation in anterior cruciate ligament reconstructive surgery. ( 0,684180341280514 )
Med Biol Eng Comput - Characterization of cellular elastic modulus using structure based double layer model. ( 0,683934621113564 )
Med Biol Eng Comput - Validation efforts and flexibilities of an eight-year-old human juvenile lumbar spine using a three-dimensional finite element model. ( 0,683668427136392 )
Med Biol Eng Comput - Continuous flow left ventricular pump support and its effect on regional left ventricular wall stress: finite element analysis study. ( 0,683492360999799 )
Wiley Interdiscip Rev Syst Biol Med - Endothelial cell motility, coordination and pattern formation during vasculogenesis. ( 0,681183481423461 )
Wiley Interdiscip Rev Syst Biol Med - Live cell single-molecule detection in systems biology. ( 0,680737863686261 )
Comput Methods Programs Biomed - Optimal bone density distributions: numerical analysis of the osteocyte spatial influence in bone remodeling. ( 0,68053867460768 )
J. Comput. Biol. - Cytopede: a three-dimensional tool for modeling cell motility on a flat surface. ( 0,679892539037605 )
Med Biol Eng Comput - Effects of skin wrinkles, age and wetness on mechanical loads in the stratum corneum as related to skin lesions. ( 0,679742138002281 )
Comput. Biol. Med. - Biomechanical simulation of various surface roughnesses and geometric designs on an immediately loaded dental implant. ( 0,67751774183362 )
Med Biol Eng Comput - Can common adhesion molecules and microtopography affect cellular elasticity? A combined atomic force microscopy and optical study. ( 0,677424756050441 )
Med Biol Eng Comput - The remodeling of alveolar bone supporting the mandibular first molar with different levels of periodontal attachment. ( 0,6771329664433 )
Int J Comput Assist Radiol Surg - Multi-scale model for investigating the electrical properties and mechanical properties of liver tissue undergoing ablation. ( 0,675768889891763 )
Comput Math Methods Med - A simplified 3D model of whole heart electrical activity and 12-lead ECG generation. ( 0,674955015657291 )
Med Biol Eng Comput - Effect of heterogeneities in the cellular microstructure on propagation of the cardiac action potential. ( 0,67266732331217 )
Med Biol Eng Comput - Biomechanics of thoracolumbar junction vertebral fractures from various kinematic conditions. ( 0,671676893328704 )
IEEE Trans Pattern Anal Mach Intell - A Histogram Transform for Probability Density Function Estimation. ( 0,671309777148773 )
Wiley Interdiscip Rev Syst Biol Med - A systems biology approach to synovial joint lubrication in health, injury, and disease. ( 0,670880292849899 )
Comput Biol Chem - Macroscopic simulations of microtubule dynamics predict two steady-state processes governing array morphology. ( 0,670507250713636 )
Comput Methods Programs Biomed - Assessment of the EEG complexity during activations from sleep. ( 0,669523394344454 )
Med Biol Eng Comput - Finite element analysis of idealised unit cell cancellous structure based on morphological indices of cancellous bone. ( 0,668177347098066 )
Comput. Biol. Med. - Modeling porous scaffold microstructure by a reaction-diffusion system and its degradation by hydrolysis. ( 0,66740085857827 )
Comput. Biol. Med. - Application of an anatomically-detailed finite element thorax model to investigate pediatric cardiopulmonary resuscitation techniques on hard bed. ( 0,664139951338497 )
Med Biol Eng Comput - Extracting the parameters of the double-dispersion Cole bioimpedance model from magnitude response measurements. ( 0,662602618509595 )
Med Biol Eng Comput - A nonlinear finite element simulation of balloon expandable stent for assessment of plaque vulnerability inside a stenotic artery. ( 0,662291339656825 )
Med Biol Eng Comput - Biomechanical analysis of traumatic mesenteric avulsion. ( 0,662121681571045 )
Int J Comput Assist Radiol Surg - Real-time simulation of the nonlinear visco-elastic deformations of soft tissues. ( 0,662026779755321 )
Med Biol Eng Comput - Backflow length predictions during flow-controlled infusions using a nonlinear biphasic finite element model. ( 0,66056784710392 )
Med Biol Eng Comput - Strain rate-dependent viscohyperelastic constitutive modeling of bovine liver tissue. ( 0,659349727920358 )
Med Biol Eng Comput - Towards determining soft tissue properties for modelling spine surgery: current progress and challenges. ( 0,657230929499903 )
Comput Methods Programs Biomed - Monte Carlo method for photon heating using temperature-dependent optical properties. ( 0,655263713113697 )
Med Biol Eng Comput - Influence of heterogeneous and anisotropic tissue conductivity on electric field distribution in deep brain stimulation. ( 0,654265362383485 )
Med Biol Eng Comput - Biomechanical modeling of brace treatment of scoliosis: effects of gravitational loads. ( 0,653728523717043 )
Med Biol Eng Comput - Estimation of the retainer height biomechanical contribution in posterior resin-bonded fixed partial dentures: a structural-thermal coupled finite element analysis. ( 0,64947455578999 )
IEEE Trans Vis Comput Graph - A Hexahedral Multigrid Approach for Simulating Cuts in Deformable Objects. ( 0,64781832462948 )
Wiley Interdiscip Rev Syst Biol Med - Reprogramming cells and tissue patterning via bioelectrical pathways: molecular mechanisms and biomedical opportunities. ( 0,64742526715896 )
Wiley Interdiscip Rev Syst Biol Med - Virtual Cell: computational tools for modeling in cell biology. ( 0,646303612180684 )
Comput Math Methods Med - The effect of inhibitory neuron on the evolution model of higher-order coupling neural oscillator population. ( 0,64627474112067 )
Int J Comput Assist Radiol Surg - Quasi-linear viscoelastic modeling of arterial wall for surgical simulation. ( 0,646036917599545 )
Comput. Biol. Med. - Finite element modelling of diseased carotid bifurcations generated from in vivo computerised tomographic angiography. ( 0,645614673556976 )
Sci Data - Parallel genome-scale loss of function screens in 216 cancer cell lines for the identification of context-specific genetic dependencies. ( 0,644476410602551 )
Med Biol Eng Comput - Modeling of the dielectric properties of trabecular bone samples at microwave frequency. ( 0,644171678954186 )
Med Biol Eng Comput - Biomechanical comparison of fusionless growth modulation corrective techniques in pediatric scoliosis. ( 0,644110227784727 )
Comput Math Methods Med - Relationship between concentration difference of different density lipoproteins and shear stress in atherosclerosis. ( 0,642398587200837 )
J Med Syst - Functional and mechanical evaluation of nerve stretch injury. ( 0,641685213642027 )
Wiley Interdiscip Rev Syst Biol Med - Stem cell genome-to-systems biology. ( 0,641239181991623 )
Comput Methods Programs Biomed - A simulation of T-wave alternans vectocardiographic representation performed by changing the ventricular heart cells action potential duration. ( 0,640750725462864 )
Comput Methods Programs Biomed - A patient specific electro-mechanical model of the heart. ( 0,640669381945291 )
Med Biol Eng Comput - Biomechanical analysis of the effects of medial meniscectomy on degenerative osteoarthritis. ( 0,640298700067052 )
Neural Comput - Deficient GABAergic gliotransmission may cause broader sensory tuning in schizophrenia. ( 0,639589277779624 )