Med Biol Eng Comput - Can common adhesion molecules and microtopography affect cellular elasticity? A combined atomic force microscopy and optical study.

Tópicos

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Resumo

The phenomenon that cells respond to chemical and topographic cues in their surroundings has been widely examined and exploited in many fields ranging from basic life science research to biomedical therapeutics. Adhesion promoting molecules such as poly-L-lysine (PLL) and fibronectin (Fn) are commonly used for in vitro cell assays to promote cell spreading/proliferation on tissue culture plastic and to enhance the biocompatibility of biomedical devices. Likewise, engineered topography is often used to guide cell growth and differentiation. Little is known about how these cues affect the biomechanical properties of cells and subsequent cell function. In this study we have applied atomic force microscopy (AFM) to investigate these biomechanical properties. In the first stage of the study we formulated a rigorous approach to quantify cellular elasticity using AFM. Operational factors, including indentation depth and speed, and mathematical models for data fitting have been systematically evaluated. We then quantified how PLL, Fn and microtopography affected cellular elasticity and the organization of the cytoskeleton. Cellular elasticity after 1 day in culture was greater on a Fn-coated surface as compared to PLL or glass. These statistically significant differences disappeared after two more days in culture. In contrast, the significantly higher elasticity associated with cells grown on micrometric grooves remained for at least 3 days. This work sheds light on the apparently simple but debatable questions: "Are engineered chemical cues eventually masked by a cell's own matrix proteins and so only exert short-term influence? Does engineered topography as well as engineered chemistry affect cell elasticity?"

Resumo Limpo

phenomenon cell respond chemic topograph cue surround wide examin exploit mani field rang basic life scienc research biomed therapeut adhes promot molecul polyllysin pll fibronectin fn common use vitro cell assay promot cell spreadingprolifer tissu cultur plastic enhanc biocompat biomed devic likewis engin topographi often use guid cell growth differenti littl known cue affect biomechan properti cell subsequ cell function studi appli atom forc microscopi afm investig biomechan properti first stage studi formul rigor approach quantifi cellular elast use afm oper factor includ indent depth speed mathemat model data fit systemat evalu quantifi pll fn microtopographi affect cellular elast organ cytoskeleton cellular elast day cultur greater fncoat surfac compar pll glass statist signific differ disappear two day cultur contrast signific higher elast associ cell grown micrometr groov remain least day work shed light appar simpl debat question engin chemic cue eventu mask cell matrix protein exert shortterm influenc engin topographi well engin chemistri affect cell elast

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