Med Biol Eng Comput - Electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells.

Tópicos

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Resumo

It has been reported previously that electric pulses of sufficiently high voltage and short duration can permeabilize the membranes of various organelles inside living cells. In this article, we describe electropermeabilization of endocytotic vesicles in B16 F1 mouse melanoma cells. The cells were exposed to short, high-voltage electric pulses (from 1 to 20 pulses, 60 ns, 50 kV/cm, repetition frequency 1 kHz). We observed that 10 and 20 such pulses induced permeabilization of membranes of endocytotic vesicles, detected by release of lucifer yellow from the vesicles into the cytosol. Simultaneously, we detected uptake of propidium iodide through plasma membrane in the same cells. With higher number of pulses permeabilization of the membranes of endocytotic vesicles by pulses of given parameters is accompanied by permeabilization of plasma membrane. However, with lower number of pulses only permeabilization of the plasma membrane was detected.

Resumo Limpo

report previous electr puls suffici high voltag short durat can permeabil membran various organell insid live cell articl describ electropermeabil endocytot vesicl b f mous melanoma cell cell expos short highvoltag electr puls puls ns kvcm repetit frequenc khz observ puls induc permeabil membran endocytot vesicl detect releas lucif yellow vesicl cytosol simultan detect uptak propidium iodid plasma membran cell higher number puls permeabil membran endocytot vesicl puls given paramet accompani permeabil plasma membran howev lower number puls permeabil plasma membran detect

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