Wiley Interdiscip Rev Syst Biol Med - Subdiffractive microscopy: techniques, applications, and challenges.

Tópicos

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Resumo

Cellular processes rely on the precise orchestration of signaling and effector molecules in space and time, yet it remains challenging to gain a comprehensive picture of the molecular organization underlying most basic biological functions. This organization often takes place at length scales below the resolving power of conventional microscopy. In recent years, several 'superresolution' fluorescence microscopic techniques have emerged that can surpass the diffraction limit of conventional microscopy by a factor of 2-20. These methods have been used to reveal previously unknown organization of macromolecular complexes and cytoskeletal structures. The resulting high-resolution view of molecular organization and dynamics is already changing our understanding of cellular processes at the systems level. However, current subdiffractive microscopic techniques are not without limitations; challenges remain to be overcome before these techniques achieve their full potential. Here, we introduce three primary types of subdiffractive microscopic techniques, consider their current limitations and challenges, and discuss recent biological applications.

Resumo Limpo

cellular process reli precis orchestr signal effector molecul space time yet remain challeng gain comprehens pictur molecular organ under basic biolog function organ often take place length scale resolv power convent microscopi recent year sever superresolut fluoresc microscop techniqu emerg can surpass diffract limit convent microscopi factor method use reveal previous unknown organ macromolecular complex cytoskelet structur result highresolut view molecular organ dynam alreadi chang understand cellular process system level howev current subdiffract microscop techniqu without limit challeng remain overcom techniqu achiev full potenti introduc three primari type subdiffract microscop techniqu consid current limit challeng discuss recent biolog applic

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