Brief. Bioinformatics - Alternative applications for distinct RNA sequencing strategies.

Tópicos

{ gene(2352) biolog(1181) express(1162) }
{ method(1557) propos(1049) approach(1037) }
{ data(1714) softwar(1251) tool(1186) }
{ research(1085) discuss(1038) issu(1018) }
{ analysi(2126) use(1163) compon(1037) }
{ sequenc(1873) structur(1644) protein(1328) }
{ compound(1573) activ(1297) structur(1058) }
{ studi(1410) differ(1259) use(1210) }
{ blood(1257) pressur(1144) flow(957) }
{ structur(1116) can(940) graph(676) }
{ use(1733) differ(960) four(931) }
{ can(774) often(719) complex(702) }
{ visual(1396) interact(850) tool(830) }
{ state(1844) use(1261) util(961) }
{ sampl(1606) size(1419) use(1276) }
{ use(2086) technolog(871) perceiv(783) }
{ health(1844) social(1437) communiti(874) }
{ imag(1947) propos(1133) code(1026) }
{ data(1737) use(1416) pattern(1282) }
{ inform(2794) health(2639) internet(1427) }
{ measur(2081) correl(1212) valu(896) }
{ imag(1057) registr(996) error(939) }
{ general(901) number(790) one(736) }
{ perform(999) metric(946) measur(919) }
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{ research(1218) medic(880) student(794) }
{ age(1611) year(1155) adult(843) }
{ cost(1906) reduc(1198) effect(832) }
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{ method(2212) result(1239) propos(1039) }
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{ health(3367) inform(1360) care(1135) }
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{ group(2977) signific(1463) compar(1072) }
{ data(3008) multipl(1320) sourc(1022) }
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{ can(981) present(881) function(850) }
{ high(1669) rate(1365) level(1280) }
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{ detect(2391) sensit(1101) algorithm(908) }

Resumo

Recent advances in RNA library preparation methods, platform accessibility and cost efficiency have allowed high-throughput RNA sequencing (RNAseq) to replace conventional hybridization microarray platforms as the method of choice for mRNA profiling and transcriptome analyses. RNAseq is a powerful technique to profile both long and short RNA expression, and the depth of information gained from distinct RNAseq methods is striking and facilitates discovery. In addition to expression analysis, distinct RNAseq approaches also allow investigators the ability to assess transcriptional elongation, DNA variance and exogenous RNA content. Here we review the current state of the art in transcriptome sequencing and address epigenetic regulation, quantification of transcription activation, RNAseq output and a diverse set of applications for RNAseq data. We detail how RNAseq can be used to identify allele-specific expression, single-nucleotide polymorphisms and somatic mutations and discuss the benefits and limitations of using RNAseq to monitor DNA characteristics. Moreover, we highlight the power of combining RNA- and DNAseq methods for genomic analysis. In summary, RNAseq provides the opportunity to gain greater insight into transcriptional regulation and output than simply miRNA and mRNA profiling.

Resumo Limpo

recent advanc rna librari prepar method platform access cost effici allow highthroughput rna sequenc rnaseq replac convent hybrid microarray platform method choic mrna profil transcriptom analys rnaseq power techniqu profil long short rna express depth inform gain distinct rnaseq method strike facilit discoveri addit express analysi distinct rnaseq approach also allow investig abil assess transcript elong dna varianc exogen rna content review current state art transcriptom sequenc address epigenet regul quantif transcript activ rnaseq output divers set applic rnaseq data detail rnaseq can use identifi allelespecif express singlenucleotid polymorph somat mutat discuss benefit limit use rnaseq monitor dna characterist moreov highlight power combin rna dnaseq method genom analysi summari rnaseq provid opportun gain greater insight transcript regul output simpli mirna mrna profil

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