Comput Biol Chem - Gene cloning, homology comparison and analysis of the main functional structure domains of beta estrogen receptor in Jining Gray goat.

Tópicos

{ sequenc(1873) structur(1644) protein(1328) }
{ gene(2352) biolog(1181) express(1162) }
{ measur(2081) correl(1212) valu(896) }
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{ studi(1119) effect(1106) posit(819) }
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{ network(2748) neural(1063) input(814) }
{ treatment(1704) effect(941) patient(846) }
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Resumo

To clarify the molecular evolution and characteristic of beta estrogen receptor (ER?) gene in Jining Gray goat in China, the entire ER? gene from Jining Gray goat ovary was amplified, identified and sequenced, and the gene sequences were compared with those of other animals. Functional structural domains and variations in DNA binding domains (DBD) and ligand binding domains (LBD) between Jining Gray goat and Boer goat were analyzed. The results indicate that the ER? gene in Jining Gray goat includes a 1584bp sequence with a complete open-reading-frame (ORF), encoding a 527 amino acid (aa) receptor protein. Compared to other species, the nucleotide homology is 73.9-98.9% and the amino acid homology is 79.5-98.5%. The main antigenic structural domains lie from the 97th aa to the 286th aa and from the 403rd aa to the 527th aa. The hydrophilicity and the surface probability of the structural domains are distributed throughout a range of amino acids. There are two different amino acids in the DBD and three different amino acids in the LBD between Jining Gray and Boer goats, resulting in dramatically different spatial structures for ER? protein. These differences may explain the different biological activities of ER? between the two goat species. This study firstly acquired the whole ER? gene sequence of Jining Gray goat with a complete open reading frame, and analyzed its gene evolutionary relationship and predicted its mainly functional structural domains, which may very help for further understanding the genome evolution and gene diversity of goat ER?.

Resumo Limpo

clarifi molecular evolut characterist beta estrogen receptor er gene jine gray goat china entir er gene jine gray goat ovari amplifi identifi sequenc gene sequenc compar anim function structur domain variat dna bind domain dbd ligand bind domain lbd jine gray goat boer goat analyz result indic er gene jine gray goat includ bp sequenc complet openreadingfram orf encod amino acid aa receptor protein compar speci nucleotid homolog amino acid homolog main antigen structur domain lie th aa th aa rd aa th aa hydrophil surfac probabl structur domain distribut throughout rang amino acid two differ amino acid dbd three differ amino acid lbd jine gray boer goat result dramat differ spatial structur er protein differ may explain differ biolog activ er two goat speci studi first acquir whole er gene sequenc jine gray goat complet open read frame analyz gene evolutionari relationship predict main function structur domain may help understand genom evolut gene divers goat er

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