Comput Biol Chem - Identification of novel splice variants and exons of human endothelial cell-specific chemotaxic regulator (ECSCR) by bioinformatics analysis.

Tópicos

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Resumo

Recent discovery of biological function of endothelial cell-specific chemotaxic regulator (ECSCR), previously known as endothelial cell-specific molecule 2 (ECSM2), in modulating endothelial cell migration, apoptosis, and angiogenesis, has made it an attractive molecule in vascular research. Thus, identification of splice variants of ECSCR could provide new strategies for better understanding its roles in health and disease. In this study, we performed a series of blast searches on the human EST database with known ECSCR cDNA sequence (Variant 1), and identified additional three splice variants (Variants 2-4). When examining the ECSCR gene in the human genome assemblies, we found a large unknown region between Exons 9 and 11. By PCR amplification and sequencing, we partially mapped Exon 10 within this previously unknown region of the ECSCR gene. Taken together, in addition to previously reported human ECSCR, we identified three novel full-length splice variants potentially encoding different protein isoforms. We further defined a total of twelve exons and nearly all exon-intron boundaries of the gene, of which only eight are annotated in current public databases. Our work provides new information on gene structure and alternative splicing of the human ECSCR, which may imply its functional complexity. This undoubtedly opens new opportunities for future investigation of the biological and pathological significance of these ECSCR splice variants.

Resumo Limpo

recent discoveri biolog function endotheli cellspecif chemotax regul ecscr previous known endotheli cellspecif molecul ecsm modul endotheli cell migrat apoptosi angiogenesi made attract molecul vascular research thus identif splice variant ecscr provid new strategi better understand role health diseas studi perform seri blast search human est databas known ecscr cdna sequenc variant identifi addit three splice variant variant examin ecscr gene human genom assembl found larg unknown region exon pcr amplif sequenc partial map exon within previous unknown region ecscr gene taken togeth addit previous report human ecscr identifi three novel fulllength splice variant potenti encod differ protein isoform defin total twelv exon near exonintron boundari gene eight annot current public databas work provid new inform gene structur altern splice human ecscr may impli function complex undoubt open new opportun futur investig biolog patholog signific ecscr splice variant

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