Comput Methods Programs Biomed - Algorithm for registration of full Scanning Laser Ophthalmoscope video sequences.

Tópicos

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Resumo

Fluorescein angiography is an established technique for examining the functional integrity of the retinal microcirculation for early detection of changes due to retinopathy. This paper describes a new method for the registration of large Scanning Laser Ophthalmoscope sequences (SLO), where the patient has been injected with a fluorescent dye. This allows the measurement of parameters such as the arteriovenous passage time. Due to the long time needed to acquire these sequences, there will inevitably be eye movement, which must be corrected prior to the application of quantitative analysis. The algorithm described here combines mutual information-based registration and landmark-based registration. The former will allow the alignment of the darkest frames of the sequence, where the dye has not still arrived to the retina, because of its ability to work with images without a preprocessing or segmentation, while the latter uses relevant features (the vessels) extracted by means of a robust creaseness operator, to get a very fast and accurate registration. The algorithm only detects rigid transformations but proves to be robust against the slight alterations derived from the eye location perspective during acquisition. Results were validated by expert clinicians.

Resumo Limpo

fluorescein angiographi establish techniqu examin function integr retin microcircul earli detect chang due retinopathi paper describ new method registr larg scan laser ophthalmoscop sequenc slo patient inject fluoresc dye allow measur paramet arterioven passag time due long time need acquir sequenc will inevit eye movement must correct prior applic quantit analysi algorithm describ combin mutual informationbas registr landmarkbas registr former will allow align darkest frame sequenc dye still arriv retina abil work imag without preprocess segment latter use relev featur vessel extract mean robust creas oper get fast accur registr algorithm detect rigid transform prove robust slight alter deriv eye locat perspect acquisit result valid expert clinician

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