Comput. Biol. Med. - A new method for gridding DNA microarrays.

Tópicos

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Resumo

In this paper, a new methodological scheme for the gridding of DNA microarrays is proposed. The scheme composes of a series of processes applied sequentially. Each DNA microarray image is pre-processed to remove any noise and the center of each spot is detected using a template matching algorithm. Then, an initial gridding is automatically placed on the DNA microarray image by 'building' rectangular pyramids around the detected spots' centers. The gridlines "move" between the pyramids, horizontally and vertically, forming this initial grid. Furthermore, a refinement process is applied composing of a five-step approach in order to correct gridding imperfections caused by its initial placement, both in non-spot cases and in more than one spot enclosure cases. The proposed gridding scheme is applied on DNA microarray images under known transformations and on real-world DNA data. Its performance is compared against the projection pursuit method, which is often used due to its speed and simplicity, as well as against a state-of-the-art method, the Optimal Multi-level Thresholding Gridding (OMTG). According to the obtained results, the proposed gridding scheme outperforms both methods, qualitatively and quantitatively.

Resumo Limpo

paper new methodolog scheme grid dna microarray propos scheme compos seri process appli sequenti dna microarray imag preprocess remov nois center spot detect use templat match algorithm initi grid automat place dna microarray imag build rectangular pyramid around detect spot center gridlin move pyramid horizont vertic form initi grid furthermor refin process appli compos fivestep approach order correct grid imperfect caus initi placement nonspot case one spot enclosur case propos grid scheme appli dna microarray imag known transform realworld dna data perform compar project pursuit method often use due speed simplic well stateoftheart method optim multilevel threshold grid omtg accord obtain result propos grid scheme outperform method qualit quantit

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