Comput. Biol. Med. - Genome-wide identification and structure-function studies of proteases and protease inhibitors in Cicer arietinum (chickpea).

Tópicos

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Resumo

CKGROUND: Proteases are a family of enzymes present in almost all living organisms. In plants they are involved in many biological processes requiring stress response in situations such as water deficiency, pathogen attack, maintaining protein content of the cell, programmed cell death, senescence, reproduction and many more. Similarly, protease inhibitors (PIs) are involved in various important functions like suppression of invasion by pathogenic nematodes, inhibition of spores-germination and mycelium growth of Alternaria alternata and response to wounding and fungal attack. As much as we know, no genome-wide study of proteases together with proteinaceous PIs is reported in any of the sequenced genomes till now.METHODS: Phylogenetic studies and domain analysis of proteases were carried out to understand the molecular evolution as well as gene and protein features. Structural analysis was carried out to explore the binding mode and affinity of PIs for cognate proteases and prolyl oligopeptidase protease with inhibitor ligand.RESULTS: In the study reported here, a significant number of proteases and PIs were identified in chickpea genome. The gene expression profiles of proteases and PIs in five different plant tissues revealed a differential expression pattern in more than one plant tissue. Molecular dynamics studies revealed the formation of stable complex owing to increased number of protein-ligand and inter and intramolecular protein-protein hydrogen bonds.DISCUSSION: The genome-wide identification, characterization, evolutionary understanding, gene expression, and structural analysis of proteases and PIs provide a framework for future analysis when defining their roles in stress response and developing a more stress tolerant variety of chickpea.

Resumo Limpo

ckground proteas famili enzym present almost live organ plant involv mani biolog process requir stress respons situat water defici pathogen attack maintain protein content cell program cell death senesc reproduct mani similar proteas inhibitor pis involv various import function like suppress invas pathogen nematod inhibit sporesgermin mycelium growth alternaria alternata respons wound fungal attack much know genomewid studi proteas togeth proteinac pis report sequenc genom till nowmethod phylogenet studi domain analysi proteas carri understand molecular evolut well gene protein featur structur analysi carri explor bind mode affin pis cognat proteas prolyl oligopeptidas proteas inhibitor ligandresult studi report signific number proteas pis identifi chickpea genom gene express profil proteas pis five differ plant tissu reveal differenti express pattern one plant tissu molecular dynam studi reveal format stabl complex owe increas number proteinligand inter intramolecular proteinprotein hydrogen bondsdiscuss genomewid identif character evolutionari understand gene express structur analysi proteas pis provid framework futur analysi defin role stress respons develop stress toler varieti chickpea

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