IEEE J Biomed Health Inform - Accurate segmentation of partially overlapping cervical cells based on dynamic sparse contour searching and GVF snake model.

Tópicos

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Resumo

Overlapping cells segmentation is one of the challenging topics in medical image processing. In this paper, we propose to approximately represent the cell contour as a set of sparse contour points, which can be further partitioned into two parts: the strong contour points and the weak contour points. We consider the cell contour extraction as a contour points locating problem and propose an effective and robust framework for segmentation of partially overlapping cells in cervical smear images. First, the cell nucleus and the background are extracted by a morphological filtering-based K-means clustering algorithm. Second, a gradient decomposition-based edge enhancement method is developed for enhancing the true edges belonging to the center cell. Then, a dynamic sparse contour searching algorithm is proposed to gradually locate the weak contour points in the cell overlapping regions based on the strong contour points. This algorithm involves the least squares estimation and a dynamic searching principle, and is thus effective to cope with the cell overlapping problem. Using the located contour points, the Gradient Vector Flow Snake model is finally employed to extract the accurate cell contour. Experiments have been performed on two cervical smear image datasets containing both single cells and partially overlapping cells. The high accuracy of the cell contour extraction result validates the effectiveness of the proposed method.

Resumo Limpo

overlap cell segment one challeng topic medic imag process paper propos approxim repres cell contour set spars contour point can partit two part strong contour point weak contour point consid cell contour extract contour point locat problem propos effect robust framework segment partial overlap cell cervic smear imag first cell nucleus background extract morpholog filteringbas kmean cluster algorithm second gradient decompositionbas edg enhanc method develop enhanc true edg belong center cell dynam spars contour search algorithm propos gradual locat weak contour point cell overlap region base strong contour point algorithm involv least squar estim dynam search principl thus effect cope cell overlap problem use locat contour point gradient vector flow snake model final employ extract accur cell contour experi perform two cervic smear imag dataset contain singl cell partial overlap cell high accuraci cell contour extract result valid effect propos method

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