IEEE Trans Image Process - Nonrigid registration of 2-D and 3-D dynamic cell nuclei images for improved classification of subcellular particle motion.

Tópicos

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Resumo

The observed motion of subcellular particles in fluorescence microscopy image sequences of live cells is generally a superposition of the motion and deformation of the cell and the motion of the particles. Decoupling the two types of movements to enable accurate classification of the particle motion requires the application of registration algorithms. We have developed an intensity-based approach for nonrigid registration of multichannel microscopy image sequences of cell nuclei. First, based on 3-D synthetic images we demonstrate that cell nucleus deformations change the observed motion types of particles and that our approach allows to recover the original motion. Second, we have successfully applied our approach to register 2-D and 3-D real microscopy image sequences. A quantitative experimental comparison with previous approaches for nonrigid registration of cell microscopy has also been performed.

Resumo Limpo

observ motion subcellular particl fluoresc microscopi imag sequenc live cell general superposit motion deform cell motion particl decoupl two type movement enabl accur classif particl motion requir applic registr algorithm develop intensitybas approach nonrigid registr multichannel microscopi imag sequenc cell nuclei first base d synthet imag demonstr cell nucleus deform chang observ motion type particl approach allow recov origin motion second success appli approach regist d d real microscopi imag sequenc quantit experiment comparison previous approach nonrigid registr cell microscopi also perform

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